Detection of DNA damage in mussels and sea urchins exposed to crude oil using comet assay

The single-cell microgel electrophoresis assay or the comet assay was used to evaluate DNA damage of dispersed crude oil on sea urchins (Strongylocentrotus droebachiensis) and mussels (Mytilus edulis L.). Sea urchins were exposed to 0.06 and 0.25 mg/L dispersed crude oil in a continuous flow system, while the mussels were exposed to 0.015, 0.06 and 0.25 mg/L dispersed crude oil. Sea urchin coelomocytes and mussel haemocytes were sampled after 4 and 5 weeks exposure, respectively. In the sea urchin coelomocytes, there was a significant concentration-related increase in the percentage of DNA in comet tail. In mussel haemocytes, there was a significantly higher percentage of DNA in comet tail for all treatments compared to the control. The responses were concentration-related up to 0.06 mg/L oil. The two highest exposure concentrations of mussels were not significantly different from each other. These results indicate that the comet assay can be used for biomonitoring of DNA damage in marine invertebrates following oil contamination.     

Co–rich Mn crusts from the Magellan Seamount cluster: the long journey through time

The Magellan seamounts began forming as large submarine shield volcanoes south of the equator during the Cretaceous. These volcanoes formed as a cluster on the small Pacific plate in a period when tectonic stress was absent. Thermal subsidence of the seafloor led to sinking of these volcanoes and the formation of guyots as the seamounts crossed the equatorial South Pacific (10–0°S) sequentially and ocean surface temperatures became too high for calcareous organisms to survive. Guyot formation was completed between about 59 and 45 Ma and the guyots became phosphatized at about 39–34 and 27–21 Ma. Ferromanganese crusts began formation as proto-crusts on the seamounts and guyots of the Magellan Seamount cluster towards the end of the Cretaceous up to 55 Ma after the formation of the seamounts themselves. The chemical composition of these crusts evolved over time in a series of steps in response to changes in global climate and ocean circulation. The great thickness of these crusts (up to 15–20 cm) reflects their very long period of growth. The high Co contents of the outer parts of the crusts are a consequence of the increasing deep circulation of the ocean and the resulting deepening of the oxygen minimum zone with time. Growth of the Co-rich Mn crusts in the Magellan Seamount cluster can be considered to be the culmination of a long journey through time.     

早第三纪济阳湖盆的形成和演变

在构造和气候的综合作用下,本区在早第三纪成为一个大型裂谷湖盆,并在沙四、沙三和沙一期因受古太平洋海泛影响而具有"近海"湖盆的特征,其演变经历了初始形成-强烈扩张-晚期扩张-收缩干涸四个阶段,构成一个完整的湖侵-湖退旋回和湖泊早期形成-中期扩张-晚期收缩三个亚旋回。     

Study on rain-runoff process in the peripheral mountainous area of the Sichuan Basin

Studies on rain-runoff process in the peripheral mountainous area of the Sichuan Basin, which is regarded as a key ecological shelter, will contribute to flood control and environmental protection for the Upper Yangtze River Basin. In two typical catchments--the Fujiang River Catchment and the Wujiang River Catchment, rainfall simulations have been conducted to study the rain-runoff processes of yellow soil and limestone soil in three types of land use--forestland, farmland and grassland. Results showed that （1） within the same rainfall process, overland flow occurs first on farmland, then on grassland, and finally on forestland; （2） soil surface coverage has a great impact on the occurrence and amount of overland flow. The runoff amount can increase 2-4 times after the coverage is removed; （3） the infiltration before the occurrence of overland flow will decrease because of higher gravel contents of soil, but it takes no effect on infiltration once overland flow becomes stable; （4） the runoff coefficient of the limestone soil forestland is greater than that of the yellow soil forest land, but less than that of the farmland; （5） three empirical infiltration models, including Horton＇ model, Kostiakov＇ model, and modified Kostiakov＇ model, were compared by using the observed results under rainfall simulation. The results showed that the Kostiakov＇ model performed better than both the Horton＇ model and modified Kostiakov model. According to the results of this research, the Kostiakov＇s model can be used to simulate rainfall infiltration when water erosion is modeled in the peripheral mountainous area of the Sichuan Basin.     

Simulation experiment on hydrocarbon generating potential of various source rocks on the northern margin of the Qaidam Basin,Qinghai, China

The northern margin of the Qaidam Basin is one of the main oil-gas exploration areas in western China, where source rocks are composed mostly of Middle and Lower Jurassic dark mud shale, carbargillite and coal. A large number of subsurface and outcrop samples differing in lithology with different types of organic matter were selected for resource evaluation, research and calculation. And among them, 13 samples were used for simulation experiment on hydrocarbon generating potential of various source rocks. At first, two kinds of heating modes were compared through simulation experiment, including single temperature-step heating and continual heating. Perhaps, the process of hydrocarbon generation and expulsion occurred naturally between a close system and an open system. In addition, the first heating mode was convenient, and all its reaction products were involved in the whole thermal evolution, and the final simulation experimental results were basically in consistency with the natural evolution trend Therefore, the first heating mode was adopted and the hydrocarbon yield of every sample was worked out. According to the type and lithology of organic matter and the hydrocarbon yield of samples for simulation experiment, hydrocarbon generation and expulsion mode with three kinds of lithology and five types of source rock has been established to provide the basis for hydrocarbon generation evaluation, research and resource calculation.     

Regional yield estimation for winter wheat with MODIS-NDVI data in Shandong, China

The significance of crop yield estimation is well known in agricultural management and policy development at regional and national levels. The primary objective of this study was to test the suitability of the method, depending on predicted crop production, to estimate crop yield with a MODIS-NDVI-based model on a regional scale. In this paper, MODIS-NDVI data, with a 250 m resolution, was used to estimate the winter wheat (Triticum aestivum L.) yield in one of the main winter-wheat-growing regions. Our study region is located in Jining, Shandong Province. In order to improve the quality of remote sensing data and the accuracy of yield prediction, especially to eliminate the cloud-contaminated data and abnormal data in the MODIS-NDVI series, the Savitzky–Golay filter was applied to smooth the 10-day NDVI data. The spatial accumulation of NDVI at the county level was used to test its relationship with winter wheat production in the study area. A linear regressive relationship between the spatial accumulation of NDVI and the production of winter wheat was established using a stepwise regression method. The average yield was derived from predicted production divided by the growing acreage of winter wheat on a county level. Finally, the results were validated by the ground survey data, and the errors were compared with the errors of agro-climate models. The results showed that the relative errors of the predicted yield using MODIS-NDVI are between −4.62% and 5.40% and that whole RMSE was 214.16 kg ha⁻¹ lower than the RMSE (233.35 kg ha⁻¹) of agro-climate models in this study region. A good predicted yield data of winter wheat could be got about 40 days ahead of harvest time, i.e. at the booting-heading stage of winter wheat. The method suggested in this paper was good for predicting regional winter wheat production and yield estimation.     

用多种策略从机载Lidar数据中提取建筑脚点

设计了一种采用多种策略的建筑脚点提取方法,针对建筑脚点提取中的两个关键步骤--数据分类和建筑脚点分割,分别提出和引入了邻近关系和r半径点密度,从仅反映单次反射的DSM数据中,直接提取出建筑表面点.     

雁南煤矿北二采区充水因素及开采影响评价

在全面分析大雁矿业集团公司雁南煤矿北二采区的水文地质条件及煤层开采矿井充水因素的基础上,计算了开采27^1号煤层时导水裂隙带发育高度．得出了北二采区各煤层工作面开采即不会受到上部砂砾含水层的影响,雁南煤矿铁路涵洞以西的胜利河冲击沟也不会受到北二采区的采动塌陷影响的结论。     

浅析旅游地图的网络化发展

旅游地图是人们旅行和导购导游的常用工具,对各地旅游业的发展具有十分重要的推动作用。本文论述了网络化旅游地图发行方式上所具有的优势,分析了当前旅游地图网络化的发展现状及存在问题,探讨了加快旅游地图网络化发展的策略与措施。     

The idea and project of the “Medium-Scale Experiment Field for Earthquake Prediction”

A brief account of the development of the research on mining earthquakes and the general situation of the Mentougou Coal Mine medium-scale experiment field for earthquake prediction and the project of monitor and prediction is given. The differences of waveforms between mining earthquakes and natural earthquakes is discussed. The magnitude-frequency distribution of the 79 000 mining earthquakes of overM _l 1. 0 from 1984 to 1995 is summarized. Finally, taking PH and PV, the principal compressive stress components of the focal mechanism of the mining earthquakes, as the criteria, analyses the stress background of the 12 large mining earthquakes.